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2023-05-12T11:24:58+00:00
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Layout
Date
Checkboxes
Protein A
Antigen Specific
Conjugation
Stabilization
Technician
*
Anti-Body
Rabbit Numbers
Batch
*
Bleed
PB 1
PB 2
PB 3
PLD
Others
Others
Layout
Column Details
Serum Volume (ml)
5 ml
Other
Other
Binding Buffer
Binding Buffer
Regular
Elution Buffer
Regular (Glycine)
Custome
Regular (Glycine)
Custom
Column Wash
OD (280nm) <=0.1
Flow rate: 2
Flow Rate 2
A Buffer
FGI A
FGI A
G Buffer
FGI G
Layout
Elution Reading
Dilution Factor(1:10)
Neutralization Buffer(1ml)
Neutralization Buffer(1ml)
Layout
OD (280 nm)
Volume (ml)
Conc. (mg/ml)
Yield (mg)
Pooled Batch Results:
Pooled Batch Results:
Flowthrough
Serum
Same Batch
Different Batches
Details
Details
Layout
Batch No.
OD (280nm)
Volume (ml)
Conc. (mg/ml)
Yield (mg)
Pooled
1
Add Row
Batch No. 2
OD (280nm) 2
Volume (ml) 2
Conc. (mg/ml) 2
Yield (mg) 2
Pooled 2
2
Add Row
Batch No. 3
OD (280nm) 3
Volume (ml) 3
Conc. (mg/ml) 3
Yield (mg) 3
Pooled 3
3
Add Row
Batch No. 4
OD (280nm) 4
Volume (ml) 4
Conc. (mg/ml) 4
Yield (mg) 4
Pooled 4
Concentration
Concentrator
10K
30K
Dilution Factor(1:20)
Layout
Initial Volume (ml)
Run Time (min)
Inner OD
Outer OD
Final Volume (ml)
Conc. (mg/ml)
Yield (mg)
Conc-1
Add Row
Initial Volume (ml) 2
Run Time (min) 2
Inner OD 2
Outer OD 2
Final Volume (ml) 2
Conc. (mg/ml) 2
Yield (mg) 2
Conc-2
Add Row
Initial Volume (ml) 3
Run Time (min) 3
Inner OD 3
Outer OD 3
Final Volume (ml) 3
Conc. (mg/ml) 3
Yield (mg) 3
Conjugation
Layout
Biotin
Biotin
Fluorescein isothiocyanate (FITC)
Fluorescein isothiocyanate (FITC)
FITC 495nm/280nm ratio
Elisa Date
Elisa File Name
Layout
Antibody Stock Conc. (mg/ml)
Conjugate OD (280nm)
Conc. (mg/ml)
Final Volume (ml)
Stabilization
Layout
Checkboxes
Conjugation
Buffer (%age)
Calculation
99% Glycerol (35)
Final Volume X 0.35
5% BSA (1)
Final Volume x 0.01
4% Sodium Azide (0.5)
Final Volume x 0.005
Affinity Purification
Affinity Purification
Volume
Volume 2
Volume 3
Layout
Total volume after Stabilization Buffer (ml)
Final concentration after Stabilization (mg/ml)
Inventory
Layout
Inventory
Aliquots (200ul)
Number
Layout
Remaining Volume (ml)
Location
Lot #
Catalog #
Remarks/ Notes
Submit
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